Journal: bioRxiv
Article Title: ME1 Programs Latent Effector Capacity and Grounds a Mathematical Model of Reversible T Cell Exhaustion
doi: 10.64898/2026.05.05.722814
Figure Lengend Snippet: (A) Schematic illustrating the generation of CD8-specific ME1 transgenic (ME1 Tg) mice. (B) Representative flow cytometry showing tdTomato expression as a surrogate for ME1 overexpression in CD8 + tumor-infiltrating lymphocytes (TILs). (C–D) Average growth curves of B16F10 tumors in control and CD8-ME1 Tg mice treated with PBS (C) or combined anti-PD-1/anti-PD-L1 antibodies (100 μg each per dose) administered every other day starting on day 6 (arrow) after tumor implantation. Tumor growth was analyzed by two-way ANOVA (D, n = 6, **P < 0.01). (E) Tumor sizes were measured at endpoint. Data were analyzed using an unpaired two-tailed t test (n = 4–8, ***P < 0.001). One of two independent experiments is shown. (F) Flow cytometric analysis of granzyme B (GZMB) protein expression in CD8 + TILs cells from B16F10 tumors in ME1 Tg and control mice on day 12. Data were analyzed using an unpaired two-tailed Student’s t test ( * P< 0.05; n = 5 mice per group).
Article Snippet: B16F10 murine melanoma cell line was purchased from ATCC (CRL-6475) and cultured using DMEM complete medium.
Techniques: Transgenic Assay, Flow Cytometry, Expressing, Over Expression, Control, Tumor Implantation, Two Tailed Test